PolymeraseChain Reaction(PCR)isanimportanttoolforthedetection ofDNA and Rl\lAwith applications across all Biotechnology / Molecular Biology research, including forensics, gene expression, identification and discovery. Traditional PCR has relied on Agorose gel-based end point detection oithe reaction, in the plateau phase.This has been time consuming, not very precise and with a degree of sample to samples variability. Real time PCR combines the amplification and detection steps into one single procedure, saving time. Real time PCR measures sample during the Exponential phase of the PCR reaction and produces results while the reaction is in progress. This achieves detection accuracy from very low initial concentrations ofstarting material. RealTime PCR is sufficiently sensitive to detect as low as zefold changes, allowing discrimination in yields that are not possibletoachievewithAgarosegels.